The researchers suggest that the findings could lead to a new approach in enhancing therapies and ultimately finding a cure for HIV.
In their quest to create treatments that could potentially eliminate HIV infection completely, scientists have been searching for all the locations where the virusA virus is a tiny infectious agent that is not considered a living organism. It consists of genetic material, either DNA or RNA, that is surrounded by a protein coat called a capsid. Some viruses also have an outer envelope made up of lipids that surrounds the capsid. Viruses can infect a wide range of organisms, including humans, animals, plants, and even bacteria. They rely on host cells to replicate and multiply, hijacking the cell's machinery to make copies of themselves. This process can cause damage to the host cell and lead to various diseases, ranging from mild to severe. Common viral infections include the flu, colds, HIV, and COVID-19. Vaccines and antiviral medications can help prevent and treat viral infections.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>virus can conceal its genetic material. In a recent study, a team of scientists from Johns Hopkins Medicine used blood samples from individuals with HIV on long-term suppressive therapy and discovered new evidence that HIV genomes can persist in a stable reservoir within circulating white blood cells known as monocytes.
Monocytes are a type of circulating immune cell with a short lifespan, that eventually develop into macrophages. Macrophages are immune cells capable of engulfing and destroying foreign elements such as viruses, bacteria, and other cells that do not belong in the host organism.
In the current research, published March 27 in Nature Microbiology, the scientists found evidence that blood samples from people with HIV undergoing long-term, standard antiretroviral therapy contained monocytes that harbor stable HIV DNADNA, or deoxyribonucleic acid, is a molecule composed of two long strands of nucleotides that coil around each other to form a double helix. It is the hereditary material in humans and almost all other organisms that carries genetic instructions for development, functioning, growth, and reproduction. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA).” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>DNA capable of infecting neighboring cells.
The scientists say the findings may provide a new direction for efforts to improve therapies and eventually cure HIV, which affects more than 34 million people worldwide, according to the World Health Organization. Current antiretroviral drugs can successfully suppress HIV to nearly undetectable levels but have not resulted in the total eradication of the virus.
“We don’t know how critical these monocytes and macrophages are to eradication of HIV, but our results suggest we should continue research efforts to understand their role in this disease,” says Janice Clements, Ph.D., professor of molecular and comparative pathobiology at the Johns Hopkins University School of Medicine.
Scientists have long known that HIV stashes its genome most often in a type of immune cell called a CD4+ T-cell. These hiding places are known as reservoirs.
“To eradicate HIV, the goal is to find biomarkers for cells that harbor the HIV genome and eliminate those cells,” says Rebecca Veenhuis, Ph.D., assistant professor of molecular and comparative pathobiology at the Johns Hopkins University School of Medicine.
To further study the role of monocytes and macrophages in circulating blood as HIV reservoirs, the Johns Hopkins-led team of scientists obtained blood samples between 2018 and 2022 from 10 men with HIV, all of them taking long-term, standard antiretroviral medications.
The researchers extracted blood cells from the samples and grew the cells in the laboratory. Typically, monocytes transform very quickly — within about three days — into macrophages, producing monocyte-derived macrophages.
All 10 men had detectable HIV DNA in their monocytes-turned-macrophages but at levels 10 times lower than those found in the men’s CD4+ T cells, the well-established HIV reservoir.
For the next phase of the research, to determine if HIV genomes were present in monocytes prior to macrophage differentiation, the team used an experimental assay to detect intact HIV genomes in monocytes. The assay was based on one that fellow Johns Hopkins scientist Robert Siliciano, M.D., Ph.D., developed in 2019 to detect the HIV genome in CD4+ T cells.
The scientists, including research associate Celina Abreu, Ph.D., used the assay on blood samples taken from another group of 30 people (eight men from the first group and 22 female participants) with HIV, also treated with standard antiretroviral therapy. The researchers found HIV DNA in the CD4+ T cells and in the monocytes of all 30 participants.
The scientists were also able to isolate HIV produced by infected monocytes from half of the research participants. The virus extracted from these cells was able to infect CD4+ T cells.
Three of the participants had their blood examined several times over the four-year study period, and each time, the scientists found HIV DNA and infectious virus produced by their monocyte-derived macrophages. “These results suggest that monocytes may be a stable reservoir of HIV,” says Clements.
In further research, the Johns Hopkins research team plans to pinpoint the subset of monocytes found to harbor HIV DNA and the source of these infected cells.
Reference: “Monocyte-derived macrophages contain persistent latent HIV reservoirs” by Rebecca T. Veenhuis, Celina M. Abreu, Pedro A. G. Costa, Edna A. Ferreira, Janaysha Ratliff, Lily Pohlenz, Erin N. Shirk, Leah H. Rubin, Joel N. Blankson, Lucio Gama, and Janice E. Clements, 27 March 2023, Nature Microbiology.
DOI: 10.1038/s41564-023-01349-3
The study was funded by the National Institutes of Health, the Johns Hopkins University National Institute of Mental Health Center for Novel Therapeutics for HIV-associated Cognitive Disorders, and the Johns Hopkins University Center for AIDS Research, an NIH-funded program.
Source: SciTechDaily
