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New DNA Evidence Rewrites Ancient American History

The origin of Assateague’s wild horses has remained a mystery for centuries, but new genetic data supports the theory that they descended from Spanish horses marooned on the barrier island. Credit: Florida Museum photo by Jeff Gage

Ancient DNA from an American domesticated horse lends credence to shipwreck folklore.

An abandoned Caribbean colony discovered centuries later and a case of mistaken identity in the archaeological record have colluded to rewrite the history of a barrier island off the shores of Virginia and Maryland.

When Nicolas Delsol, a postdoctoral researcher at the Florida Museum of Natural History, set out to analyze ancient DNADNA, or deoxyribonucleic acid, is a molecule composed of two long strands of nucleotides that coil around each other to form a double helix. It is the hereditary material in humans and almost all other organisms that carries genetic instructions for development, functioning, growth, and reproduction. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA).” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>DNA recovered from cow bones discovered in archaeological sites, these seemingly unrelated threads were woven together. Delsol wanted to know how cattle were domesticated in the Americas, and he discovered the answer in the genetic information preserved in centuries-old teeth. However, they also held a surprise.

“It was a serendipitous finding,” he said. “I was sequencing mitochondrial DNA from fossil cow teeth for my Ph.D. and realized something was very different with one of the specimens when I analyzed the sequences.”

Horse Tooth

This tooth is all that remains from one of the first horses introduced to the Americas, and its DNA is helping to rewrite the history of one of the best-known horse breeds in the United States: The Chincoteague pony. Credit: Florida Museum photo by Jeff Gage

That’s because the specimen in question, a portion of an adult molar, was a horse tooth rather than a cow tooth. According to recent research published in PLOS ONE, the DNA extracted from the tooth is also the oldest ever sequenced for a domesticated horse from the Americas.

The tooth was discovered during the excavation of one of Spain’s oldest colonized settlements. Puerto Real, located on the island of Hispaniola, was founded in 1507 and served as the final port of call for ships traveling from the Caribbean for decades. In the 16th century, however, widespread piracy and the expansion of illegal commerce drove the Spanish to concentrate their influence elsewhere on the island, and people were ordered to abandon Puerto Real in 1578. The abandoned settlement was razed by Spanish authorities the following year.

The ruins of the once-bustling harbor were unexpectedly uncovered in 1975 by a medical missionary called William Hodges. Between 1979 and 1990, archaeologists headed by Florida Museum distinguished research curator Kathleen Deagan excavated the site.

Horse Skull

Nicolas Delsol was originally sequencing ancient DNA from cow teeth preserved in archaeological sites when he realized one of his specimens actually belonged to a horse. Credit: Florida Museum photo by Jeff Gage

Horse fossils and related artifacts are very uncommon at Puerto Real and comparable time-period sites, whereas cow remains are plentiful. According to Delsol, this lopsided ratio is mostly attributed to the way Spanish colonialists valued their livestock.

“Horses were reserved for individuals of high status, and owning one was a sign of prestige,” he said. “There are full-page descriptions of horses in the documents that chronicle the arrival of [Hernán] Cortés in Mexico, demonstrating how important they were to the Spanish.”

In contrast, cows were used as a source of meat and leather, and their bones were regularly discarded in communal waste piles called middens. But one community’s trash is an archaeologist’s treasure, as the refuse from middens often confers the clearest glimpse into what people ate and how they lived.

The specimen’s biggest surprise wasn’t revealed until Delsol compared its DNA with that of modern horses from around the world. Given that the Spanish brought their horses from the Iberian Peninsula in southern Europe, he expected horses still living in that region would be the closest living relatives of the 500-year-old Puerto Real specimen.

Instead, Delsol found its next of kin over 1,000 miles north of Hispaniola, on the island of Assateague off the coast of Maryland and Virginia. Feral horses have roamed freely across the long stretch of barrier island for hundreds of years, but exactly how they got there has remained a mystery.

According to the National Park Service, which manages the northern half of Assateague, the likeliest explanation is that the horses were brought over in the 1600s by English colonists from the mainland in an attempt to evade livestock taxes and fencing laws. Others believe the feral herds descended from horses that survived the shipwreck of a Spanish galleon and swam to shore, a theory popularized in the 1947 children’s novel “Misty of Chincoteague.” The book was later adapted to film, helping spread the shipwreck legend to an even wider audience.

Until now, there has been little evidence to support either theory. Proponents of the shipwreck theory claim it would be unlikely that English colonists would lose track of valuable livestock, while those in favor of an English origin of the herds point to the lack of sunken vessels nearby and the omission of feral horses in historical records of the region.

The results of the DNA analysis, however, unequivocally point to Spanish explorers as being the likeliest source of the horses on Assateague, Delsol explained.

“It’s not widely reported in the historical literature, but the Spanish were exploring this area of the mid-Atlantic pretty early on in the 16th century. The early colonial literature is often patchy and not completely thorough. Just because they don’t mention the horses doesn’t mean they weren’t there.”


The feral herds on Assateague weren’t the only horses to revert back to their wild heritage after arriving in the Americas. Colonists from all over Europe brought with them horses of various breeds and pedigrees, some of which bucked their bonds and escaped into the surrounding countryside.

Today, the U.S. Bureau of Land Management estimates there are roughly 86,000 wild horses across the country, most of which are located in western states, such as Nevada and Utah. Delsol hopes that future ancient DNA studies will help decode the complex history of equine introductions and migrations that occurred over the last several centuries and offer a clearer understanding of today’s diversity of wild and domesticated horses.

Reference: “Analysis of the earliest complete mtDNA genome of a Caribbean colonial horse (Equus caballus) from 16th-century Haiti” by Nicolas Delsol, Brian J. Stucky, Jessica A. Oswald, Elizabeth J. Reitz, Kitty F. Emery and Robert Guralnick, 27 July 2022, PLOS ONE.
DOI: 10.1371/journal.pone.0270600

The study was funded by the National Science Foundation. 

Source: SciTechDaily