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New Enzyme Has Remarkable Capacity To Help Break Down Chemical Building Block of PET Plastic

The TPADO enzyme. Credit: Rita Clare, Montana State University

Scientists who helped to pioneer the use of enzymes to eat plastic have taken an important next step in developing nature-based solutions to the global plastics crisis.

Scientists who helped to pioneer the use of enzymes to eat plastic have taken an important next step in developing nature-based solutions to the global plastics crisis.

They have characterized an enzyme that has the remarkable capacity to help break down terephthalate (TPA), one of the chemical building blocks of polyethylene terephthalate (PET) plastic, which is used to make single-use drinks bottles, clothing, and carpets.

The research, which is published in The Proceedings of the National Academy of Sciences (PNAS), was co-led by Professor Jen DuBois, Montana State University, and Professor John McGeehan from the University of PortsmouthEstablished in 1992, the University of Portsmouth is a public university in the city of Portsmouth, Hampshire, England. Prior to reaching university status, the school was known as Portsmouth Polytechnic from 1969 until 1992 and can trace its history back to 1870 as the Portsmouth and Gosport School of Science and Art.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>University of Portsmouth, who in 2018 led the international team that engineered a natural enzyme that could break down PET plastic. The enzymes (PETase and MHETase) break the PET polymer into the chemical building blocks ethylene glycol (EG) and TPA. This new research describes the next steps, specifically for managing TPA.

John McGeehan

Professor John McGeehan. Credit: Stefan Venter, UPIX Photography

Professor DuBois said: “While EG is a chemical with many uses – it’s part of the antifreeze you put into your car, for example – TPA does not have many uses outside of PET, nor is it something that most bacteria can even digest. However, the Portsmouth team revealed that an enzyme from PET-consuming bacteria recognizes TPA like a hand in a glove. Our group at MSU then demonstrated that this enzyme, called TPADO, breaks down TPA and pretty much only TPA, with amazing efficiency.”

With more than 400 million tons of plastic waste produced each year, the overwhelming majority of which ends up in landfills, it is hoped this work will open the door to improve bacterial enzymes, such as TPADO. This will help tackle the challenge of plastic pollution and develop biological systems that can convert waste plastic into valuable products.

Professor McGeehan, who is the Director of the University’s Centre for Enzyme Innovation, said: “The last few years have seen incredible advances in the engineering of enzymes to break down PET plastic into its building blocks. This work goes a stage further and looks at the first enzyme in a cascade that can deconstruct those building blocks into simpler molecules. These can then be utilized by bacteria to generate sustainable chemicals and materials, essential making valuable products out of plastic waste.

“Using powerful X-ray at the Diamond Light SourceDiamond Light Source is the UK’s national synchrotron. It works like a giant microscope, harnessing the power of electrons to produce bright light that scientists can use to study anything from fossils to jet engines to viruses and vaccines. The machine accelerates electrons to near light speeds so that they give off light 10 billion times brighter than the sun.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>Diamond Light Source, we were able to generate a detailed 3D structure of the TPADO enzyme, revealing how it performs this crucial reaction. This provides researchers with a blueprint for engineering faster and more efficient versions of this complex enzyme.”

Reference: “Biochemical and structural characterization of an aromatic ring–hydroxylating dioxygenase for terephthalic acidAny substance that when dissolved in water, gives a pH less than 7.0, or donates a hydrogen ion.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>acid catabolism” by 21 March 2022, Proceedings of the National Academy of Sciences.

The study was undertaken as part of the BOTTLE Consortium, an international collaboration between the US and UK, bringing together researchers from across a wide range of scientific areas to tackle plastic recycling and upcycling.

Source: SciTechDaily