Powerful New Tool Can Peer Into the Vast Genetic Library Inside of Your Cells

The human genome can be thought of as a massive library, containing over 20,000 different “instruction manuals”: your genes. For example, there are genes which contain information to build a brain cell, a skin cell, a white blood cell, and so on. There are even genes that contain information about regulating the genome itself—like books that explain how to organize a library. The ability to regulate gene expression—in other words, the cell’s ability to turn various constellations of genes on or off—is the basis of why different cells (such as a muscle cell or a brain cell) have different forms and functions.

For any library to be useful to a reader, it needs to be organized in an easily searchable way. For example, all the books pertaining to world history may be on one shelf, whereas the cookbooks may be in an entirely different section of the library. In a cellular nucleus, there is over six feet of genetic material packed into a space 50 times smaller than the width of a human hair. How is the “library” in the nucleus organized? When a cell needs to regulate certain genes, how does the cellular machinery find the right ones amongst 20,000 others?

A new paper from the laboratory of Mitchell Guttman, professor of biology, uses a powerful new tool that can peer into the world of the cell’s genetic material (DNADNA, or deoxyribonucleic acid, is a molecule composed of two long strands of nucleotides that coil around each other to form a double helix. It is the hereditary material in humans and almost all other organisms that carries genetic instructions for development, functioning, growth, and reproduction. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA).”>DNA and RNARibonucleic acid (RNA) is a polymeric molecule similar to DNA that is essential in various biological roles in coding, decoding, regulation and expression of genes. Both are nucleic acids, but unlike DNA, RNA is single-stranded. An RNA strand has a backbone made of alternating sugar (ribose) and phosphate groups. Attached to each sugar is one of four bases—adenine (A), uracil (U), cytosine (C), or guanine (G). Different types of RNA exist in the cell: messenger RNA (mRNA), ribosomal RNA (rRNA), and transfer RNA (tRNA).”>RNA) in order to find answers to these questions.

Led by former Guttman lab graduate student Sofia Quinodoz (PhD ’20)—now a Hanna Gray postdoctoral fellow at Princeton UniversityFounded in 1746, Princeton University is a private Ivy League research university in Princeton, New Jersey and the fourth-oldest institution of higher education in the United States. It provides undergraduate and graduate instruction in the humanities, social sciences, natural sciences, and engineering.”>Princeton University—the team found that molecules of non-coding RNA are responsible for establishing “compartments” within the nucleus and shepherding in key molecules to precise regions in the genome. Noncoding RNA are molecules that do not encode for proteins, and instead have an array of functions that are often still mysterious to biologists. In the library analogy, non-coding RNA molecules act as the “shelves” that organize different groups of genes and the machinery that interacts with them.

Understanding how genetic material is organized spatially is a crucial part of understanding the basic workings of life. Dysfunction within the nucleus is a hallmark of many diseases, including cancer, neurodegenerative disorders, and others.

The research was made possible by a powerful tool developed in the Guttman laboratory that enables detailed views of the RNA world, called RD-SPRITE (RNA and DNA Split-Pool Recognition of Interactions by Tag Extension). In essence, RD-SPRITE works by tagging molecules of RNA and DNA with minuscule unique barcodes based on their locations; analyzing the barcodes can then tell you which molecules were at which positions within the cell.

“This tool is something I’ve dreamed of since I was a grad student. It’s remarkable that Sofia was able to make this happen,” says Guttman. “It changes what we can look at in the RNA world. It’s like developing a new microscope; you can start looking at things you could never see before. This discovery about RNA and organization is the tip of the iceberg in terms of what we are able to start finding in these data.”

The team plans to use RD-SPRITE to compare the spatial organization of the nucleus between healthy cells and disease cell types, to understand how gene expression and the physical structure of the nucleus may be affected in disease states.

Reference: “RNA promotes the formation of spatial compartments in the nucleus” by Sofia A. Quinodoz, Joanna W. Jachowicz, Prashant Bhat, Noah Ollikainen, Abhik K. Banerjee, Isabel N. Goronzy, Mario R. Blanco, Peter Chovanec, Amy Chow, Yolanda Markaki, Jasmine Thai, Kathrin Plath and Mitchell Guttman, 4 November 2021, Cell.
DOI: 10.1016/j.cell.2021.10.014

The paper is titled “RNA promotes the formation of spatial compartments in the nucleus.” Sofia Quinodoz is the paper’s first author along with co-second authors Caltech postdoctoral scholar Joanna Jachowicz, graduate student Prashant Bhat, and former postdoctoral scholar Noah Ollikainen. In addition to Guttman, other coauthors include former graduate student Abhik Banerjee, graduate student Isabel Goronzy, research scientist Mario Blanco, former postdoctoral scholar Peter Chovanec, senior research scientist Amy Chow, Yolanda Markaki of UCLA, former research technician assistant Jasmine Thai, and Kathrin Plath of UCLA. Funding was provided by the Howard Hughes Medical Institute, the National Science Foundation, the National Institutes of Health, the UCLA-Caltech Medical Scientist Training Program, the American Cancer Society, the Division of Biology and Biological Engineering at Caltech, the National Heart Lung and Blood Institute, and the USCFounded in 1880, the University of Southern California is one of the world’s leading private research universities. It is located in the heart of Los Angeles.“>USC MD/PhD program.