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Chemists Reveal How Tau Proteins Form Alzheimer’s Disease Brain Tangles

MIT chemists have used nuclear magnetic resonance (NMR) spectroscopy to reveal how two different forms of the Tau protein mix to form the tangles seen in the brains of Alzheimer’s patients. Credit: Aurelio Dregni/Nadia El-Mammeri/Hong Lab at MIT

Two types of tau proteins mix together in a nearly random way to generate the tangles seen in the brains of people with Alzheimer’sAlzheimer's disease is a disease that attacks the brain, causing a decline in mental ability that worsens over time. It is the most common form of dementia and accounts for 60 to 80 percent of dementia cases. There is no current cure for Alzheimer's disease, but there are medications that can help ease the symptoms.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>Alzheimer’s Disease.

One of the hallmarks of Alzheimer’s disease is the presence of neurofibrillary tangles in the brain. These tangles, made of tau proteins, impair neurons’ ability to function normally and can cause the cells to die.

A new research study from MITMIT is an acronym for the Massachusetts Institute of Technology. It is a prestigious private research university in Cambridge, Massachusetts that was founded in 1861. It is organized into five Schools: architecture and planning; engineering; humanities, arts, and social sciences; management; and science. MIT's impact includes many scientific breakthroughs and technological advances. Their stated goal is to make a better world through education, research, and innovation.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>MIT chemists has revealed how two types of tau proteins, known as 3R and 4R tau, mix together to form these tangles. The researchers found that the tangles can recruit any tau protein in the brain, in a nearly random way. This feature may contribute to the prevalence of Alzheimer’s disease, the researchers say.

“Whether the end of an existing filament is a 3R or 4R tau protein, the filament can recruit whichever tau version is in the environment to add onto the growing filament. It is very advantageous for the Alzheimer’s disease tau structure to have that property of randomly incorporating either version of the protein,” says Mei Hong, an MIT professor of chemistry.

Hong is the senior author of the study, which was recently published in the journal Nature CommunicationsNature Communications is a peer-reviewed, open access, multidisciplinary, scientific journal published by Nature Research. It covers the natural sciences, including physics, biology, chemistry, medicine, and earth sciences. It began publishing in 2010 and has editorial offices in London, Berlin, New York City, and Shanghai. ” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>Nature Communications. MIT graduate student Aurelio Dregni and postdoc Pu Duan are the lead authors of the paper.

Molecular mixing

In the healthy brain, tau functions as a stabilizer of microtubules in neurons. Each tau protein is made up of either three or four “repeats,” each consisting of 31 amino acidAny substance that when dissolved in water, gives a pH less than 7.0, or donates a hydrogen ion.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>acid residues. Abnormal versions of either 3R or 4R tau proteins can contribute to a variety of diseases.

Chronic traumatic encephalopathy, caused by repetitive head trauma, is linked to abnormal accumulation of both 3R and 4R tau proteins, similar to Alzheimer’s disease. However, most other neurodegenerative diseases that involve tau feature abnormal versions of either 3R or 4R proteins, but not both.

In Alzheimer’s disease, tau proteins begin to form tangles in response to chemical modifications of the proteins that interfere with their normal function. Each tangle consists of long filaments of 3R and 4R tau proteins, but it wasn’t known exactly how the proteins combine at the molecular level to generate these long filaments.

One possibility that Hong and her colleagues considered was that the filaments might be made of alternating blocks of many 3R tau proteins or many 4R tau proteins. Or, they hypothesized, individual molecules of 3R and 4R tau might alternate.

The researchers set out to explore these possibilities using nuclear magnetic resonance (NMR) spectroscopy. By labeling 3R and 4R tau proteins with carbon and nitrogen isotopes that can be detected with NMR, the researchers were able to calculate the probabilities that each 3R tau protein is followed by a 4R tau and that each 4R tau is followed by a 3R tau protein in a filament.

To produce their filaments, the researchers began with abnormal tau proteins taken from postmortem brain samples from Alzheimer’s patients. These “seeds” were added to a solution containing equal concentrations of normal 3R and 4R tau proteins, which were recruited by the seeds to form long filaments.

To the researchers’ surprise, their NMR analysis showed that the assembly of these 3R and 4R tau proteins in these seeded filaments was nearly random. A 4R tau was about 40 percent likely to be followed by a 3R tau, while a 3R tau was a little more than 50 percent likely to be followed by a 4R tau. Overall, 4R proteins made up 60 percent of the Alzheimer’s disease tau filament, even though the pool of available tau proteins was evenly divided between 3R and 4R. Within the human brain, 3R and 4R tau proteins are also found in roughly equal amounts.

This type of assembly, which the researchers call “fluent molecular mixing,” may contribute to the prevalence of Alzheimer’s disease, compared to diseases that involve only 4R or 3R tau proteins, Hong says.

“Our interpretation is that this would favor the spread and the growth of the toxic Alzheimer’s disease tau conformation,” she says.

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Toxic effects

Working with collaborators at the University of Pennsylvania School of Medicine, led by Professor Virginia Lee, the researchers showed that the tau filaments they generated in the lab have a structure very similar to those seen in human patients with Alzheimer’s disease, but they do not resemble filaments grown exclusively from normal tau proteins.

The tau filaments that they generated also replicated the toxic effects of Alzheimer’s tangles, forming aggregates in the dendrites and axons of mouse neurons grown in a lab dish.


The current paper focused mainly on the structure of the rigid inner core of the filaments, but the researchers now hope to further study the structure of the floppier protein segments that extend out from this core. “We would like to figure out just how this protein goes from a healthy and intrinsically disordered state to this toxic, misfolded, and beta-sheet rich state in Alzheimer’s disease brains,” Hong says.

Reference: “Fluent molecular mixing of Tau isoforms in Alzheimer’s disease neurofibrillary tangles” by Aurelio J. Dregni, Pu Duan, Hong Xu, Lakshmi Changolkar, Nadia El Mammeri, Virginia M.-Y. Lee and Mei Hong, 27 May 2022, Nature Communications.
DOI: 10.1038/s41467-022-30585-0

The research was funded by the National Institutes of Health and the BrightFocus Foundation.

Source: SciTechDaily