New research has discovered that the protein dynein is critical in breast cancer cell movement, providing a new target for treatments that could “paralyze” cancer cells, potentially offering a non-destructive alternative to chemotherapy. Though clinical applications are still forthcoming, this breakthrough offers promising implications for the future of personalized cancer therapy.
The most lethal aspect of any cancer is metastasis, which is the spread of cancer cells throughout the body. Groundbreaking research led by Penn State has uncovered for the first time the mechanisms that may enable breast cancer cells to invade healthy tissues. This pivotal discovery reveals that a motor protein known as dynein is instrumental in propelling cancer cell movement in models of soft tissue, presenting novel clinical targets for hindering metastasis and potentially revolutionizing cancer treatment.
“This discovery marks a paradigm shift in many ways,” said Erdem Tabdanov, assistant professor of pharmacology at Penn State and a lead co-corresponding author on the study, recently published in the journal Advanced Science. “Until now, dynein has never been caught in the business of providing the mechanical force for cancer cell motility, which is their ability to move themselves. Now we can see that if you target dynein, you could effectively stop motility of those cells and, therefore, stop metastatic dissemination.”
Collaborative Efforts and Model Systems
The project began as a collaboration between Penn State’s Department of Chemical Engineering and Penn State’s College of Medicine, before growing into a multi-institution partnership with researchers at the University of Rochester Medical Center, Georgia Institute of Technology, Emory University, and the U.S. Food and Drug Administration.
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Human breast cancer cells are seen migrating within a 3D model for healthy soft tissue, designed by Amir Sheikhi of Penn State. The microgels are invisible to avoid visual interference with the cells. The nuclei of the cells are green. Credit: Erdem Tabdanov/Penn State
The researchers used live microscopy to watch the migration of live breast cancer cells in two different systems modeled after the human body. The first system, a two-dimensional network of collagen fibers, revealed how cancer cells move through an extracellular matrix that surrounds tumors and showed that dynein was key to the movement of cancer cells.
The second system was a three-dimensional model developed by a team led by Amir Sheikhi, Dorothy Foehr Huck, and J. Lloyd Huck Early Career Chair in Biomaterials and Regenerative Engineering and assistant professor of chemical engineering and biomedical engineering at Penn State.
Discoveries in Three-Dimensional Models
The second system was designed to mimic soft tissue using a network of microscopic hydrogel particles or microgels linked together in tumor-like shapes. Like in the two-dimensional model, the researchers found in the three-dimensional model that dynein was “indispensable” in the spread or metastasis of cancer cells.
“Using these three-dimensional models that partially mimic a tumor, we discovered that if we block the dynein, the cancer cells cannot effectively move and infiltrate solid tissues,” Sheikhi said. “In both models, we found that dynein is extremely important for cell locomotion, which suggests a whole new method for cancer management. Instead of killing the cancer cells with radiation or chemotherapy, we are showing how to paralyze them. This is great news because you don’t really have to kill the cells, which is a harsh approach that targets both cancerous and healthy cells. Instead, you just have to stop the cancer cells from moving.”
A human breast cancer cell, adenocarcinoma MDA-MB-231, demonstrates metastatic-like adhesion, spreading and migrating in a collagen matrix designed to mimic soft tissue. New research led by Penn State reveals for the first time the mechanics behind how breast cancer cells may invade healthy tissues. The discovery, showing that a motor protein called dynein powers the movement of cancer cells in soft tissue models, offers new clinical targets against metastasis and has the potential to fundamentally change how cancer is treated. Credit: Courtesy Erdem Tabdanov
Tabdanov explained that cell “paralysis” could prove to be an effective treatment strategy for cancer compared to chemotherapeutic treatments, because after surgical removal of the main tumor, it could prevent the cancer from spreading without damaging healthy tissues and cells.
“The trick with chemotherapy is to kill the cancer cells slightly faster than the rest of the body — it’s a race against time,” Tabdanov said. “Chemotherapy causes a lot of damage to the body’s normal, healthy tissues while it is busy killing the cancer. If we instead contained the cancer, stopped it in its tracks, we could keep the healthy parts of the body healthy.”
Future Prospects
The researchers noted that any potential clinical treatment is still far off — as they have yet to run human or animal trials. Sheikhi has filed multiple patents related to his team’s platform and plans to use the technology to study a myriad of diseases, including other cancers.
“We are very excited about this collaboration with the Penn State College of Medicine, and our labs are working closely on other projects,” Sheikhi said. “I think these platforms could one day enable personalized medicine and personalized treatment for cancer and, hopefully, many other diseases.”
Reference: “Dynein-Powered Cell Locomotion Guides Metastasis of Breast Cancer” by Yerbol Tagay, Sina Kheirabadi, Zaman Ataie, Rakesh K. Singh, Olivia Prince, Ashley Nguyen, Alexander S. Zhovmer, Xuefei Ma, Amir Sheikhi, Denis Tsygankov and Erdem D. Tabdanov, 19 September 2023, Advanced Science.
DOI: 10.1002/advs.202302229
Other authors on the paper are Yerbol Tagay of Penn State College of Medicine; Sina Kheirabadi and Zaman Ataie of Penn State’s Department of Chemical Engineering; Rakesh Singh of the University of Rochester Medical Center; Denis Tsygankov of Georgia Institute of Technology and Emory University; and Olivia Prince, Ashley Nguyen, Alexander Zhovmer and Xuefei Ma of the U.S. Food and Drug Administration.
Startup funds from the Department of Pharmacology at Penn State College of Medicine, the Meghan Rose Bradley Foundation, the National Science Foundation, the National Institutes of HealthThe National Institutes of Health (NIH) is the primary agency of the United States government responsible for biomedical and public health research. Founded in 1887, it is a part of the U.S. Department of Health and Human Services. The NIH conducts its own scientific research through its Intramural Research Program (IRP) and provides major biomedical research funding to non-NIH research facilities through its Extramural Research Program. With 27 different institutes and centers under its umbrella, the NIH covers a broad spectrum of health-related research, including specific diseases, population health, clinical research, and fundamental biological processes. Its mission is to seek fundamental knowledge about the nature and behavior of living systems and the application of that knowledge to enhance health, lengthen life, and reduce illness and disability.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>National Institutes of Health, and the U.S. Food and Drug Administration supported this work.
Source: SciTechDaily
