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Exciting Possibilities: New Species of Microalgae Discovered

Green lines with dots show a pure culture of Medakamo hakoo streaked onto an agar medium. Study co-author Emeritus Professor Tsuneyoshi Kuroiwa first discovered the algae when the water in his home goldfish tank turned green after he added medaka fish (Japanese rice fish), and he decided to look at samples under his home microscope. However, where the algae originated from – the water, fish, or somewhere else – is still unknown. Credit: 2023 Sachihiro Matsunaga

The discovery of ultrasmall microalgae in a home aquarium could have multiple useful applications.

Researchers from the University of Tokyo have uncovered a new speciesA species is a group of living organisms that share a set of common characteristics and are able to breed and produce fertile offspring. The concept of a species is important in biology as it is used to classify and organize the diversity of life. There are different ways to define a species, but the most widely accepted one is the biological species concept, which defines a species as a group of organisms that can interbreed and produce viable offspring in nature. This definition is widely used in evolutionary biology and ecology to identify and classify living organisms.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>species of microalgae, named Medakamo hakoo, during a DNADNA, or deoxyribonucleic acid, is a molecule composed of two long strands of nucleotides that coil around each other to form a double helix. It is the hereditary material in humans and almost all other organisms that carries genetic instructions for development, functioning, growth, and reproduction. Nearly every cell in a person’s body has the same DNA. Most DNA is located in the cell nucleus (where it is called nuclear DNA), but a small amount of DNA can also be found in the mitochondria (where it is called mitochondrial DNA or mtDNA).” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>DNA analysis of water from a home aquarium. With its unique DNA sequence not found in any previous records, this species stands out as the smallest known freshwater green algae. Its remarkable ability to be stably cultivated at high densities opens up exciting possibilities for its use in producing valuable products for both food and industrial purposes.

If you have ever encountered seaweed, navigated through vegetation in a stream, or cleared a murky green aquarium, you are familiar with algae. These diverse aquatic organisms, which come in various shapes, colors, and sizes, thrive on water, light, and nutrients. Microalgae, a type of algae that is ultrasmall and invisible to the naked eye, play a crucial role in the Earth’s ecosystem as they serve as the foundation of all aquatic food chains.

They have attracted particular attention from researchers and businesses for their ability to capture carbon dioxide, their use as a biofuel, as an alternative source of protein, and more. There are tens of thousands of types of microalgae, which continue to thrive in unexpected places.

This microscopic fluorescent image of a Medakamo hakoo shows a chloroplast (red), a nucleus (green), and a cytoplasm (blue) in the alga’s cell. The white scale bar indicates 500 nanometers (0.0005 millimeters). Credit: 2023 Tsuneyoshi Kuroiwa

“We were very surprised to discover a new species of microalgae in just a regular home aquarium,” said Professor Sachihiro Matsunaga from the Graduate School of Frontier Sciences. “Alga were taken from the water and cultured one by one. The DNA of the alga was fluorescently stained and microscopically observed to find the one with the least amount of DNA per cell. We then sequenced the DNA of that alga and compared it to the DNA of other algae. The results did not match the DNA of any previously reported algae, indicating that it was a new species, and we named it Medakamo hakoo (M. hakoo).”

Microalgae are made up of relatively few genes, and this uncomplicated form makes them useful for researchers trying to identify what roles different genes play and how they could be used. Of the tens of thousands of known microalgae, many remain uncharacterized. Thanks to this latest study, we now know that not only is this a new species, but it also has the smallest known genome of any freshwater algae, as well as other useful qualities.

M. hakoo contains only one mitochondrion (for producing energy) and one chloroplast (which contains chlorophyll and creates food through photosynthesisPhotosynthesis is how plants and some microorganisms use sunlight to synthesize carbohydrates from carbon dioxide and water.” data-gt-translate-attributes=”[{“attribute”:”data-cmtooltip”, “format”:”html”}]”>photosynthesis), whereas normal plant cells contain multiple mitochondria and chloroplasts. This indicates that it is a green alga with an extremely simple cell structure,” explained Matsunaga. “From our research, we have also speculated that it has an unprecedented DNA structure and a new gene regulatory system. Its cell cycle is also strongly synchronized with the day and night cycle, which is key to effective, stable bioproduction. Due to these inherent qualities and extremely small size, M. hakoo can be effectively cultured at high cell density, making it possible to mass produce substances such as highly functional foods, cosmetics, and bio-fuel at a low cost.”

The researchers plan to continue to explore the potential applications for M. hakoo, both in the lab and the wider world. “Aquatic green algae are the originating organisms of today’s land plants. Thanks to this research, we can better understand the minimum number of genes required for an organism to evolve and thrive in diverse environments, which we will continue to study,” said Matsunaga. “In the future, I would like to find ways to collaborate and create useful substances from the mass cultivation of M. hakoo.”

Reference: “Genomic analysis of an ultrasmall freshwater green alga, Medakamo hakoo” by Shoichi Kato, Osami Misumi, Shinichiro Maruyama, Hisayoshi Nozaki, Yayoi Tsujimoto-Inui, Mari Takusagawa, Shigekatsu Suzuki, Keiko Kuwata, Saki Noda, Nanami Ito, Yoji Okabe, Takuya Sakamoto, Fumi Yagisawa, Tomoko M. Matsunaga, Yoshikatsu Matsubayashi, Haruyo Yamaguchi, Masanobu Kawachi, Haruko Kuroiwa, Tsuneyoshi Kuroiwa and Sachihiro Matsunaga, 23 January 2023, Communications Biology.
DOI: 10.1038/s42003-022-04367-9

Funding: MXT/JSPS KAKENHI funding, JST-CREST and JST-OPERA grants.

Source: SciTechDaily